Unlocking the secrets of biomacromolecules — CryoEM at atomic resolution

Cryo-EM Single Particle Analysis (SPA)

Leveraging our advanced CryoEM (CryoTEM) facility and expert team, Shuimu Biosciences (水木未来) excels in Single Particle Analysis (SPA), delivering precise high-resolution structures for drug discovery and AIDD. With over 2000 SPA projects — including antibody structures, GPR75, GPCRs, and membrane proteins — and resolutions reaching down to 1.4Å, we lead the way in advancing structural biology and structure-based drug design.

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Applications

✓High-resolution 3D structures of macromolecules

✓3D structures of small molecules with target proteins in drug discovery

✓Structural characterization of antibodies and interactions with antigens

✓Structural characterization of lipid nanoparticles, liposomes, AAV, and other viral vectors

✓Structural biology application in PROTAC

Workflow

Sample Reception & QC

Receive and evaluate sample quality using negative stain EM and DLS

Grid Preparation

Optimize vitrification conditions using automated grid preparators

Data Collection

Automated high-throughput data collection on Titan Krios G3i/G4

Image Processing

Motion correction, CTF estimation, particle picking, 2D/3D classification

High-res Refinement

Bayesian polishing, high-resolution refinement, and map sharpening

Model Building & Validation

Atomic model building, refinement, and comprehensive validation report

Why Choose Shuimu?

Advanced Cryo-EM Facility

The highest specs microscopes & advanced computing platform for high-quality structure determination

Cutting Edge Scientist Team

A team of PhD scientists from top institutions, expertised in structural biology, protein science and computation

Profound Experience

2000+ cryo-EM projects experience, from membrane proteins to antigen-antibody complexes

Resolution Pursuit

1200+ protein structures determined at resolution better than 3.5Å: the best at 1.4Å and the smallest being 51kDa

Structures Solved by Shuimu

Apoferritin structure at 1.8Å resolution, 501.25kDa — Cryo-EM SPA by Shuimu Biosciences

GPCR receptor complex with allosteric agonist and G protein at 3.2Å — Cryo-EM SPA by Shuimu

SARS-CoV-2 S-Trimer vaccine candidate at 2.6Å resolution, 526.77kDa — Shuimu Biosciences

Proteinase K structure at 1.5Å resolution, 29.05kDa — MicroED by Shuimu Biosciences

FUS LC RAC molecular structure at 0.65Å resolution, 0.66kDa — MicroED by Shuimu

Acetaminophen structure at 0.65Å resolution, 151.16Da — MicroED by Shuimu Biosciences

Explore Other Services

Cryo-ET Tomography (TOMO)

Cryo-electron tomography (CryoTEM / TOMO) for in-situ structural biology, enabling visualization of macromolecular complexes in their native cellular environment.

EM Characterization

Comprehensive sample screening and quality assessment using advanced cryo-EM techniques to ensure optimal data collection.

Data Collection

High-throughput cryo-EM data collection services on 300kV and 120kV instruments with automated processing pipelines.

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