From gene to purified protein

Protein Production

In-house protein expression laboratory supporting bacterial, insect, and mammalian cell expression systems for high-quality protein production.

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Applications

Bacterial expression (E. coli)
Insect cell expression (Sf9, High Five)
Mammalian cell expression (HEK293, CHO)
Protein purification and characterization

Workflow

1

Construct Design

Gene optimization and construct design

2

Expression Screening

Test multiple constructs and conditions

3

Scale-up Production

Large-scale expression in optimal system

4

Purification

Affinity, ion exchange, and size exclusion chromatography

5

Quality Control

SDS-PAGE, DLS, mass spectrometry verification

Why Choose Shuimu?

Multiple Expression Systems

Bacterial, insect, and mammalian systems for diverse protein types

End-to-End Service

From gene synthesis to purified, characterization-ready protein

Structures Solved by Shuimu

Apoferritin structure at 1.8Å resolution, 501.25kDa — Cryo-EM SPA by Shuimu Biosciences
GPCR receptor complex with allosteric agonist and G protein at 3.2Å — Cryo-EM SPA by Shuimu
SARS-CoV-2 S-Trimer vaccine candidate at 2.6Å resolution, 526.77kDa — Shuimu Biosciences
Proteinase K structure at 1.5Å resolution, 29.05kDa — MicroED by Shuimu Biosciences
FUS LC RAC molecular structure at 0.65Å resolution, 0.66kDa — MicroED by Shuimu
Acetaminophen structure at 0.65Å resolution, 151.16Da — MicroED by Shuimu Biosciences

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